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Full Length Research Paper

Antibacterial Activity Of Ocimum gratissimum Leaves Extract On Escherichia Coli O157

 *Chijioke A. Nsofor , Chikezie U.N and Azuwuike C.O

Department of Biotechnology, Federal University of Technology, Owerri, Imo State Nigeria

  Corresponding Authors email:nsoforac@gmail.com
 

Accepted  3 October 2014

The aim of this  study was  to investigate the antibacterial activity of Ocimum gratissimum leaves  extract against  Escherichia coli O157. A serial two fold dilution of Ocimum gratissimum leaves extract was inoculated with fixed amount (1*10⁷ cfu) of E. coli. The turbidity corresponding to the bacterial growth in various tubes was measured as optical density (O.D.) value at 600nm wavelength with the help of spectrophotometer. The result showed  that there is antibacterial activity of the leaves extract against E. coli O157. The maximum activity of extract was observed at 1:256 dilution of extract and minimum inhibitory concentration was calculated at 1:512 dilution. All the groups containing extract showed significantly lower colony forming units (C.F.U) as compared to control positive. However, lowest count was observed at 1:256 dilution of 5% extract with maximum percent inhibition of bacterial growth (39.98%) as compared to all other groups. It is conclude that the Ocimum gratissimum leaves possess good antibacterial activity and so its growth inhibitory effect against E. coli can be used to develop alternative herbal medicines.

 Keywords: Ocimum gratissimum, leaves extract, Escherichia coli 0157

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Palgo Journal of Medicine and Medical Science

© Palgo Journals 2014